WR33-115, the story (4 of 4)

• WR33 belongs to a large protein domain family with homologues in several eukaryotic genomes, Homo sapiens, Mus musculis and Drosophila melanogaster

• None of the members of this strongly conserved protein domain family has a characterized biological function

• The Bos Taurus homologue (38% identity) is characterized as “brain specific protein P25”

• WR33-180 (190 residues long) was studied previously

  • Residues 20-120 have largely alpha-helical structure (5 helices)
  • Residues 125-180 adopt largely disordered structure
  • Assignment process very difficult due to severe overlap

• Several constructs were produced to get rid of the tail and make amenable working with it while keeping 3D structure.

  • WR33-109, WR33-113, WR33-115, WR33-117
  • Based on HSQC screening, WR33-115 was selected
  • Fast and reliable way of mapping domains

Notes: