remarks To be called after reading molecular structure and coordinates {* Turn on the energy flag for positional ncs restraints.*} flags include ncs end {* Restrain only those residues not in lattice contacts.*} ncs restraints group equi (segid 1 and not hydrogen and not (resid 58:66 or resid 90:94 or resid 127:129 or resid 156:165 or resid 325:328) ) equi (segid 3 and not hydrogen and not (resid 58:66 or resid 90:94 or resid 127:129 or resid 156:165 or resid 325:328) ) equi (segid 5 and not hydrogen and not (resid 58:66 or resid 90:94 or resid 127:129 or resid 156:165 or resid 325:328) ) weight-ncs=300. sigb=1.0 end group equi (segid 2 and not hydrogen and not (resid 10:12 or resid 26:28)) equi (segid 4 and not hydrogen and not (resid 10:12 or resid 26:28)) equi (segid 6 and not hydrogen and not (resid 10:12 or resid 26:28)) {* Put looser restraints on the second group.*} weight-ncs=200. sigb=1.5 end ? {*Print the NCS relations when starting.*} end