Now that we know the orientation and position of both molecules, we can carry out rigid-body refinement of all eight domains of the two Fabs. In this particular case, the R value after rigid-body refinement was 41% at 8--3 Å resolution. The following example file also creates a new molecular structure file, which consists of a combination of molecules A and B.
This completes the structure solution of the 26-10 Fab. Now we are ready for SA-refinement.